Journal: Scientific Reports

Article Title: Long noncoding RNA DHRS4 antisense RNA 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a competitive endogenous RNA mechanism

doi: 10.1038/s41598-025-87246-7

Figure Lengend Snippet: Regulation of DHRS4-AS1 on OS cell proliferation and apoptosis in vitro. (A) Differential expression of DHRS4-AS1 in human osteoblast cell line and three OS cell lines, *Represent comparison with the hFOB1.19 group. ( B) Overexpression expression of DHRS4-AS1 in MG63, *Represent comparison with the BC group. ( C) DHRS4-AS1 silencing models in 143B cells, *Represent comparison with the BC group. ( D) CCK-8 results showing proliferation level over 4 consecutive days for MG63 cells in each group, *Represent comparison with the NC group. ( E) Proliferation level for 143B cells in each group, *Represent comparison with the NC group. ( F) Tunel results showing apoptosis level for MG63 cells in each group. ( G) Tunel results showing apoptosis level for 143B cells in each group. ( H) Quantitative analysis of apoptosis rate of MG63 cells in different groups, *Represent comparison with the NC group. ( I) Quantitative analysis of apoptosis rate of 143B cells in different groups, *Represent comparison with the NC group. ( J) Apoptosis-related protein levels of MG63 and 143B cells in each group were determined using western blot. ( K) Quantitative analysis of apoptosis-related protein in MG63 cells, *Represent comparison with the NC group. ( L) Quantitative analysis of apoptosis-related protein in 143B cells, *Represent comparison with the NC group. * p <0.05, ** p <0.01, *** p <0.001, the scale is 100 μm. BC and NC represent Blank Control and Negative Control respectively.

Article Snippet: The 143B human OS cell line was purchased from iCell Bioscience Inc, Shanghai iCell-h231). hFOB1.19 cells were cultured in DMEM/F12 (SH30126.01; HyClone; Cytiva) containing 10% FBS (10099; Thermo Fisher Scientific, Inc.) and 0.3 mg/ml G418 (PB180125; Procell Life Science & Technology Co., Ltd.) with 5% CO 2 at 37 ̊C.

Techniques: In Vitro, Quantitative Proteomics, Comparison, Over Expression, Expressing, CCK-8 Assay, TUNEL Assay, Western Blot, Control, Negative Control

Journal: Scientific Reports

Article Title: Long noncoding RNA DHRS4 antisense RNA 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a competitive endogenous RNA mechanism

doi: 10.1038/s41598-025-87246-7

Figure Lengend Snippet: DHRS4-AS1 directly interacts with miR-362-5p and represses its expression. ( A) The expression level of miR-362-5p in osteosarcoma cell lines and the human osteoblast cell line, *Represent comparison with the Hfob1.19 group. ( B) The expression level of miR-362-5p in MG63 cells was increased after transfection with miR-362-5p mimic, *Represent comparison with the mm group. ( C) After co-transfection with DHRS4-AS1 and miR-362-5p mimic, DHRS4-AS1 reversed the high expression of miR-362-5p in MG63 cells, *Represent comparison with the mNC group, # represents comparison with the mm group. ( D-E) Expression levels of apoptosis-related proteins in MG63 cells, *Represent comparison with the mNC group, # represents comparison with the mm group. ( F) cck8 assay was performed to detect the proliferation and apoptosis levels of MG63 cells after co-transfection with DHRS4-AS1 and miR-362-5p mimic, *Represent comparison with the mNC group, # represents comparison with the mm group. ( G) Quantification of apoptosis rate in MG63 cells after co-transfection with DHRS4-AS1 and miR-362-5p mimic, *Represent comparison with the mNC group, # represents comparison with the mm group. ( H) Tunel detection of apoptosis in MG63 cells after co-transfection with DHRS4-AS1 and miR-362-5p mimic. * p <0.05, ** p <0.01, *** p <0.001, ### p <0.001, the scale is 100 μm. mm represent miR-362-5p mimic group. mNC represent the mimic Negative Control group. D + mm represents the DHRS4-AS1 + miR-362-5p mimic group, indicating that both DHRS4-AS1 and miR-362-5p are overexpressed. D + mNC represents the DHRS4-AS1 + mimic Negative Control group, indicating that DHRS4-AS is overexpressed but not expressing miR-362-5p. BC represents Blank Control group.

Article Snippet: The 143B human OS cell line was purchased from iCell Bioscience Inc, Shanghai iCell-h231). hFOB1.19 cells were cultured in DMEM/F12 (SH30126.01; HyClone; Cytiva) containing 10% FBS (10099; Thermo Fisher Scientific, Inc.) and 0.3 mg/ml G418 (PB180125; Procell Life Science & Technology Co., Ltd.) with 5% CO 2 at 37 ̊C.

Techniques: Expressing, Comparison, Transfection, Cotransfection, CCK-8 Assay, TUNEL Assay, Negative Control, Control

Journal: Scientific Reports

Article Title: Long noncoding RNA DHRS4 antisense RNA 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a competitive endogenous RNA mechanism

doi: 10.1038/s41598-025-87246-7

Figure Lengend Snippet: MiR-362-5p mediates the proliferation and apoptosis of OS cells through NPEPPS. (A-B) The expression level of NPEPPS in osteosarcoma cell lines and the human osteoblast cell line, *Represent comparison with the Hfob1.19 group. ( C-D) NPEPPS was overexpressed in MG63 OS cells, *Represent comparison with the pN group. ( E-F) the expression level of NPEPPS and poptosis related protein in MG63 cells after cotransfected miR-362-5p mimic and NPEPPS, *Represent comparison with the NC group. ( G) The Tunel assay was used to successfully detect apoptosis in MG63 cells after co-transfection with miR-362-5p mimetics and NPEPPS. ( H) The CCK-8 assay was employed to determine the proliferation and apoptosis levels of MG63 cells co-transfected with miR-362-5p mimetics and NPEPPS, *Represent comparison with the NC group. ( I) The apoptosis rate in MG63 cells co-transfected with miR-362-5p mimic and NPEPPS was quantified, *Represent comparison with the NC group, # represents comparison with the pN group. * p <0.05, ** p <0.01, *** p <0.001, # p <0.01, ## p <0.001, ### p <0.001, the scale is 100 μm. NC represents Negative Control group. BC represents Blank Control group. mm represent miR-362-5p mimic group. pN represents the pCMV NPEPPS group, which overexpresses NPEPPS using the pCMV NPEPPS vector. mm + pN represents the miR-362-5p mimetic + pCMV NPEPP group, indicating the overexpression of both miR-362-5p and NPEPPS using miR-362-5p mimetic transfection and pCMV NPEP vector.

Article Snippet: The 143B human OS cell line was purchased from iCell Bioscience Inc, Shanghai iCell-h231). hFOB1.19 cells were cultured in DMEM/F12 (SH30126.01; HyClone; Cytiva) containing 10% FBS (10099; Thermo Fisher Scientific, Inc.) and 0.3 mg/ml G418 (PB180125; Procell Life Science & Technology Co., Ltd.) with 5% CO 2 at 37 ̊C.

Techniques: Expressing, Comparison, TUNEL Assay, Cotransfection, CCK-8 Assay, Transfection, Negative Control, Control, Plasmid Preparation, Over Expression

Journal: PLoS ONE

Article Title: Generating a Non-Integrating Human Induced Pluripotent Stem Cell Bank from Urine-Derived Cells

doi: 10.1371/journal.pone.0070573

Figure Lengend Snippet: A. UCs from healthy (UC-012) and diseased donors (listed in and ). B. Left: EdU imaging of representative UC. Right: EdU positive percentages of 5 UCs. Error bars are standard deviation of the mean, n = 3. C. Phase contrast and fluorescent photographs of UC-012 and UC-015 electroporation with episomal plasmid pCEP4-EGFP and cultured for 24 h in UC medium. D. Growth curves of UC-012 and UC-015 in UC medium, defined medium E8 and mTeSR1, respectively. *** indicates P <0.001. E. Schematic representation of iPS cell generation. The defined medium can be either mTeSR1 or E8. F. Different reprogramming factor combinations for iPS cell generation of UCs and skin fibroblasts from the same donor. −: failure, +: success. G. Representative phase contrast photographs of a successful iPS cell generation process: (1) Emerging iPS cell colony generating by our optimized method in E8 from UC-012 at different time points. (2)A picked colony. (3) The established iPS cell line from this colony. P8: passage 8. H. Top: AP staining of iPS cells generated from UC-012, UC-015 and UC-041 in 6 well plates. I. Bottom: reprogramming efficiencies of UC-012, UC-015 and UC-041. n = 3.

Article Snippet: Defined medium mTeSR1 (STEMCELL) or E8 medium (DMEM/F12 (Hyclone) +2% ITS (Gibco)+Vc (70 µg/ml, Sigma)+bFGF (100 ng/ml, Invitrogen)+Tgfβ1 (2 ng/ml, PeproTech) ) was changed daily during generation.

Techniques: Imaging, Standard Deviation, Electroporation, Plasmid Preparation, Cell Culture, Staining, Generated

Journal: STAR Protocols

Article Title: Generating Patient-Derived Gliomas within Cerebral Organoids

doi: 10.1016/j.xpro.2019.100008

Figure Lengend Snippet:

Article Snippet: DMEM/F12 Medium , Hyclone , Cat# SH30126.01; N/A.

Techniques: Recombinant, Concentration Assay, Selection